| Front | Other than iPSC, what other techniques do we use to retrieve stem cells and what are their strengths and weaknesses |
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| Back | IVF spare embryos: hormone treatment stimulates egg production in woman, eggs are taken from woman's ovaries to be fertilised by sperm cells in culture dish, fertilised eggs placed in incubator for 48 hours and implanted in uterus or frozen for future stem cell use (s: make use of otherwise wasted embryos and cells are pluripotent so better than adult stem cells; w: embryo deserves a right to life, organs grown with these stem cells can be rejected and pluripotency isn't as useful as totipotency) Therapeutic cloning/somatic nuclear transfer: egg cell enucleated, nucleus removed from a somatic body cell and inserted into the egg cell which is induced into an embryo so the stem cells can be harvested (s: stem cells have identical DNA to patient so organs won't be rejected and research can use animal eggs and human nuclei; w: there is a limited supply of eggs and the cells are pluripotent so not as useful as totipotent cells |
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