| Front | How do you carry out the root tip squash in order to observe the stages of mitosis to see the anaphase is the quickest stage of mitosis. Give the safety concerns and precautions as well |
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| Back | Cut off the root tip (meristem is the area of active cell division), add HCl acid to break down the middle lamella to separate the cells, add acetic orcein stain to make the chromosomes visible under a microscope, gently heat it to intensify the stain, squash the root tip under a coverslip to make a one cell thick layer, observe the stages of mitosis under the microscope and the low percentage of cells in anaphase proves that anaphase is the shortest stage Safety: stain or acid could get on skin or clothes so wear lab coat and goggles, could cut yourself with the scalpel when cutting the meristem off so cut away from you, could break coverslip or get cut when squashing the root tip so place a paper towel under the thumb when doing so |
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