The ELISA test is used to detect the presence of antigens or antibodies, either directly or indirectly, and is used in HIV testing before Western blot confirmation.
La prueba ELISA se utiliza para detectar la presencia de antígenos o anticuerpos, ya sea directamente (detectando antígenos) o indirectamente (detectando complejos antígeno-anticuerpo).
| Front | What is ELISA test used for? |
|---|---|
| Back | To detect presence of Ag or Ab Direct ELISA → Detect Ag using Ab bound to color-changing enzyme Indirect ELISA → detect Ag-Ab complex using antibody bound to color-changing enzyme Suspected HIV → ELISA → ELISA (+) → western blot (confirmatory test) |
Tags: microbiology
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