Question

If a culture inoculated to a density of 5 x 102 at time 0 and has a generation and lag time of 10 minutes, how many cells will be there in 40 minutes?

Accepted Answer

Each cell divides into two at each generation following the single lag phase

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At the end of 10 minutes there are 5 x10²

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At the end of 20 minutes there are 10x10²

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At the end of 20 minutes there are 20x10²

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At the end of 40 minutes there are 40x10²

Cells experience exponential growth

Front	If a culture inoculated to a density of 5 x 10² at time 0 and has a generation and lag time of 10 minutes, how many cells will be there in 40 minutes?
Back	Each cell divides into two at each generation following the single lag phase ↓ At the end of 10 minutes there are 5 x10² ↓ At the end of 20 minutes there are 10x10² ↓ At the end of 20 minutes there are 20x10² ↓ At the end of 40 minutes there are 40x10² Cells experience exponential growth

End Minutes Time Generation Lag Cells Culture Inoculated