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A-Level Biology OCR A

Advantages Disadvantages Covalent/Ionic Cost Varies Enzymes Substrate Describe

Covalent/ionic immobilization offers strong enzyme binding and good accessibility, with pH and substrate concentration having little effect. However, it can be costly and may modify the active site, reducing effectiveness.

Covalent/ionic immobilization offers strong enzyme binding and good accessibility, with pH and substrate concentration having little effect. However, it can be costly and may modify the active site, reducing effectiveness.

Front Describe the advantages and disadvantages of immobilisation using covalent/ionic bonding.
Back Covalent/ionic immobilisation:

Advantages
  • Cost varies
  • Enzymes strongly bound and unlikely to be lost
  • Enzymes very accessible to substrate
  • pH and substrate conc often have little effect on enzyme activity
Disadvantages
  • Cost varies
  • Active site of enzyme may be modified in process, making it less effective

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