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Advantages Disadvantages Product Contamination Higher Rate Expensive Describe

The flashcard outlines the benefits of immobilized enzymes, such as easier product separation, reusability, and increased stability. It also details the drawbacks, including slower reaction rates, higher setup costs, and potential expense if the matrix becomes contaminated.

The flashcard outlines the benefits of immobilized enzymes, such as easier product separation, reusability, and increased stability. It also details the drawbacks, including slower reaction rates, higher setup costs, and potential expense if the matrix becomes contaminated.

Front Describe the advantages and disadvantages of using immobilised enzymes.
Back
Advantages
  • Product easily separated - prevents contamination of product with enzymes (less downstream processing therefore cheaper)
  • Enzmyes can be reused and are immediately available for reuse - cheaper
  • Increased stability of enzyme - tolerant to higher temps and broader pH changes
Disadvantages
  • Lower rate of reaction (altered or blocked active sites/reduced collision rate from substrates)
  • Higher set up time/costs - more complex running process - more expensive
  • Contamination of matrix would require replacement - very expensive


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