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Colorimeter Quantitative Test Reducing Filter Benedict's Transmission Glucose

Front Explain how a colorimeter can be used in a quantitative test for reducing sugars.
Back Quantitative test for reducing sugars
  • Place filter into colorimeter
  • Calibrate colorimeter using distilled water
  • Perform normal Benedict's test (with quantitative grade Benedict's solution)
  • Filter off the solutions to remove precipitate
  • Measure % transmission for each of the solution of glucose
  • Plot a calibration curve of transmission against known glucose conc
  • Using the calibration curve, use transmisison % of unknown to find out the conc

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