A-Level Biology OCR A
Colorimeter Quantitative Test Reducing Filter Benedict's Transmission Glucose
| Front |
Explain how a colorimeter can be used in a quantitative test for reducing sugars. |
| Back |
Quantitative test for reducing sugars- Place filter into colorimeter
- Calibrate colorimeter using distilled water
- Perform normal Benedict's test (with quantitative grade Benedict's solution)
- Filter off the solutions to remove precipitate
- Measure % transmission for each of the solution of glucose
- Plot a calibration curve of transmission against known glucose conc
- Using the calibration curve, use transmisison % of unknown to find out the conc
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A-Level Biology OCR A