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Colorimeter Quantitative Test Reducing Filter Benedict's Transmission Glucose

A colorimeter can be used quantitatively for reducing sugars by performing Benedict's test, measuring light transmission of filtered solutions, plotting a calibration curve with known concentrations, and using it to determine the concentration of an unknown sample.

Para una prueba cuantitativa de azúcares reductores con un colorímetro, se realiza la prueba de Benedict, se mide la transmisión de luz de las muestras filtradas, se crea una curva de calibración con concentraciones conocidas y se usa para determinar la concentración de la muestra desconocida.

Front Explain how a colorimeter can be used in a quantitative test for reducing sugars.
Back Quantitative test for reducing sugars
  • Place filter into colorimeter
  • Calibrate colorimeter using distilled water
  • Perform normal Benedict's test (with quantitative grade Benedict's solution)
  • Filter off the solutions to remove precipitate
  • Measure % transmission for each of the solution of glucose
  • Plot a calibration curve of transmission against known glucose conc
  • Using the calibration curve, use transmisison % of unknown to find out the conc

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