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Marker Gene Genetically Explain Identify Organisms Successfully Engineered

Marker genes, such as antibiotic resistance, are integrated into plasmids alongside the gene of interest. When the gene of interest disrupts the marker gene, the absence of the marker's characteristic indicates successful genetic engineering in the bacteria.

Marker genes, such as antibiotic resistance, are integrated into plasmids alongside the gene of interest. When the gene of interest disrupts the marker gene, the absence of the marker's characteristic indicates successful genetic engineering in the bacteria.

Front Explain how marker genes can be used to identify organisms that have been successfully genetically engineered.
Back Marker genes:
  • Plasmids are genetically engineered to contain marker gene (eg antibiotic resistance)
  • Gene of interest is spliced into middle of marker gene so it ceases to function
  • If gene is inserted successfully, bacteria will not show the characteristic for the marker gene so able to kno which have the recombinant DNA and which dont

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