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A-Level Biology OCR A

Monoclonal Hcg Antibody Mouse Fused Hybridoma Describe Produced

Monoclonal antibodies for hCG are produced by immunizing a mouse with hCG, isolating its antibody-producing B-cells, fusing these with myeloma cells to form hybridomas, and then cloning these hybrid cells.

Monoclonal antibodies to hCG are produced by injecting a mouse with hCG, isolating antibody-producing B-cells, fusing them with myeloma cells to create hybridomas, and then culturing these rapidly multiplying clones.

Front Describe how monoclonal antibodies to hCG are produced.
Back Producing hCG monoclonal antibodies:
  • Mouse is injected with hCG so it makes the appropriate antibody
  • B-cells that make the required antibody are then removed from the spleen of the mouse & fused with a myeloma
  • New fused cell - hybridoma
  • Each hybridoma reproduces rapidly, resulting in clone of millions of cells that produce the desired antibody
  • Monoclonal antibodies are collected, purified and used in variety of ways

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