| Text | Required Practical 1: Investgiation into the effect of a named variable on the rate of an enzyme-controlled reaction ● Take two flat bottomed tubes. ● Add 5cm3 of milk to each tube. ● Add 5cm3 of distilled water to one tube- this control will indicate the absence of enzyme activity. ● Add 5cm3 of hydrochloric acid to the other- this control indicates the colour of a completely hydrolysed sample. 2. Take three test tubes and measure 5cm3 milk into each. Place in water bath at 10°C for 5 minutes to equilibrate. 3. Add 5cm3 trypsin to each test tube simultaneously and start the timer immediately. 4. Record how long it takes for the milk samples to completely hydrolyse and become colourless. 5. Repeat steps 2-3 at temperatures of 20°C, 30°C, 40°C and 50°C. 6. Find the mean time for the milk to be hydrolysed at each temperature and use this to work out the rate of reaction. |
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