| Front | Describe the procedure for ELISA test |
|---|---|
| Back | 1. Monoclonal antibody fixed to well 2. Sample added which includes antigen 3.Wash 4.Add first monoclonal antibody to well, complementary to antigen therefore they bind 5. Wash 6. Add 2nd monoclonal antibody which is complementary to the first antibody. This antibody has an enzyme attached. 7. Wash 8. Add substrate which changes colour when broken down by enzyme |
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