| Front | Process of comparison of genetic fingerprints |
|---|---|
| Back | Sample of DNA obtained e.g. from persons blood, saliva PCR used to make many copies of area of DNA that contain the VNTR- primers used to bind either side of these repeats and so the whole repeat is amplified. You end up with DNA fragments where the length corresponds to number of repeats Fluorescent tag added to all DNA fragments so they can be viewed under UV light DNA fragments undergo electrophoresis DNA mixture placed into well in a slab of gel covered in buffer solution that conducts electricity Electrical current passed through the gel- DNA fragments are negatively charged, so they move towards the positive electrode Small DNA fragments move faster and travel further through the gel, so the DNA fragments separate according to size DNA fragments are viewed as bands under UV light Two genetic fingerprints are compared, if both fingerprints have a band at the same location on the gel it means they have the same number of VNTR's at that place. |
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