The process of using DNA probes involves fragmenting DNA, separating fragments via electrophoresis, transferring them to a membrane, and incubating with a labeled probe; hybridization is then detected using a visualization method like UV light for fluorescent labels.
Using DNA probes involves digesting DNA into fragments, separating them by electrophoresis, transferring them to a membrane, and incubating with a labeled probe. Hybridization is detected by visualizing the label (e.g., fluorescent band under UV light).
| Front | Process of using DNA probes |
|---|---|
| Back | Sample of DNA digested into fragments using restriction enzymes and separated using electrophoresis Separated DNA fragments are transferred onto nylon membrane and incubated with fluorescently labelled DNA probe If allele is present the DNA probe will hybridise Membrane then exposed to UV light and if the gene is present there will be a fluorescent band |
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