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Dna Fragments Separated Membrane Probe Present Process Sample

Front Process of using DNA probes
Back Sample of DNA digested into fragments using restriction enzymes and separated using electrophoresis

Separated DNA fragments are transferred onto nylon membrane and incubated with fluorescently labelled DNA probe

If allele is present the DNA probe will hybridise

Membrane then exposed to UV light and if the gene is present there will be a fluorescent band

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