| Front | PCR (polymerase chain reaction) |
|---|---|
| Back | A reaction mixture is set up that contains the DNA sample, free nucleotides, primers and DNA polymerase DNA mixture heat to 95 degrees to break the hydrogen bonds between the 2 strands of DNA The mixture is then cooled between 60 to 70 degrees so that the primers can bind to the strands Reaction mixture heated to 72 degrees so the polymerase can work DNA polymerase lines up free floating DNA nucleotides alongside each template strand, specific base pairing means new complementary strands are formed Two new copies of the fragment DNA are formed and one cycle of PCR is complete |
Learn with these flashcards. Click next, previous, or up to navigate to more flashcards for this subject.
Next card: Crops transformed recombinant dna technology agriculture give higher
Previous card: Vitro cloning copies dna fragments made living organism
Up to card list: AQA Biology Flashcards