Transcription involves DNA strand separation, using one strand as a template, RNA polymerase aligning complementary RNA nucleotides, joining them to form mRNA, and then reforming hydrogen bonds and detaching RNA polymerase at a stop signal.
Transcripția implică separarea filamentele ADN, utilizarea unei filamente ca matriță, alinierea nucleotidelor ARN complementare de către ARN polimerază, unirea acestora pentru a forma o moleculă de ARNm, iar apoi refacerea legăturilor de hidrogen și detașarea ARN polimerazei la semnalul de oprire.
| Front | What are the steps of transcription |
|---|---|
| Back | 1. DNA Helicase attaches to DNA and hydrogen bonds between DNA strands break and the DNA molecule uncoils. DNA helicase requires energy in the form of ATP 2. One of the strands acts as a template strand 3.RNA polymerase aligns free-floating RNA nucleotides with an exposed base. Complementary base pairing occurs the, therefore mRNA strand is a complimentary copy of DNA template. 4.RNA polymerase joins the RNA nucleotides forming mRNA molecule 5. RNA polymerase moves along DNA separating the strands. 6. Hydrogen bonds between uncoiled strands of DNA reforms once RNA polymerase has passed, and DNA is converted into a double helix. 7. When RNA polymerase reaches a stop signal, it stops making mRNA and detaches from DNA. In Eukaryotes the mRNA moves out the nucleus through nuclear pore and attaches to the ribosome for translation. |
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