Restriction endonucleases cut DNA at specific palindromic sequences, sometimes creating sticky ends (short single-stranded overhangs) that enable complementary base pairing with other DNA fragments.
Restriction endonucleases cut DNA at specific palindromic recognition sites. This process can leave 'sticky ends' (unpaired bases) that facilitate binding to other DNA fragments with complementary sticky ends.
| Front | Using restriction endonucleases to produce DNA fragments |
|---|---|
| Back | DNA have palindromic sequences of nucleotide and consist of antiparallel base pairs. Different restriction endonucleases cut a different specific recognition sequences due to shape of recognition sequence is complementary to enzymes active site. If recognition sequences are present at either side of the DNA fragment you can use restriction endonucleases to separate it from the rest of the DNA The DNA sample is incubated with specific restriction endonucleases which cut the DNA fragment out via a hydrolysis reaction. Sometimes the cut leaves sticky ends which can be used to bind the DNA fragment to another piece of DNA that has sticky ends with complementary sequences |
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