Question

Using restriction endonucleases to produce DNA fragments

Accepted Answer

DNA have palindromic sequences of nucleotide and consist of antiparallel base pairs.
Different restriction endonucleases cut a different specific recognition sequences due to shape of recognition sequence is complementary to enzymes active site.
If recognition sequences are present at either side of the DNA fragment you can use restriction endonucleases to separate it from the rest of the DNA
The DNA sample is incubated with specific restriction endonucleases which cut the DNA fragment out via a hydrolysis reaction.
Sometimes the cut leaves sticky ends which can be used to bind the DNA fragment to another piece of DNA that has sticky ends with complementary sequences

Dna Restriction Endonucleases Sequences Cut Recognition Fragment Specific