Question

Insertion of DNA fragment into a vector

Accepted Answer

Vector DNA cut open using the same restriction endonucleases that was used to isolate the DNA fragment, so the sticky ends of the vector are complementary to the sticky ends of the DNA fragment containing the gene

Vector DNA and DNA fragment are mixed together with DNA ligase which joins the sticky ends of the DNA fragments to the sticky ends of the Vector DNA

Front	Insertion of DNA fragment into a vector
Back	Vector DNA cut open using the same restriction endonucleases that was used to isolate the DNA fragment, so the sticky ends of the vector are complementary to the sticky ends of the DNA fragment containing the gene Vector DNA and DNA fragment are mixed together with DNA ligase which joins the sticky ends of the DNA fragments to the sticky ends of the Vector DNA

Dna Vector Sticky Ends Fragment Insertion Cut Open