Inserting a DNA fragment into a vector requires cutting both with the same restriction enzyme to create complementary sticky ends, followed by ligation using DNA ligase.
DNA fragment insertion into a vector involves cutting both the vector and the fragment with the same restriction enzyme, then mixing them with DNA ligase to join complementary sticky ends.
| Front | Insertion of DNA fragment into a vector |
|---|---|
| Back | Vector DNA cut open using the same restriction endonucleases that was used to isolate the DNA fragment, so the sticky ends of the vector are complementary to the sticky ends of the DNA fragment containing the gene Vector DNA and DNA fragment are mixed together with DNA ligase which joins the sticky ends of the DNA fragments to the sticky ends of the Vector DNA |
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