Scanning electron microscopes generate 3D images by scanning a specimen's surface with an electron beam and detecting emitted secondary electrons, requiring specimens to be metal-stained and viewed in a vacuum.
Scanning electron microscopes (SEM) scan a surface with an electron beam, detecting secondary electrons to create a 3D image. Specimens are stained with heavy metals and viewed in a vacuum to prevent electron scattering.
| Front | How does scanning electron microscope work |
|---|---|
| Back | A beam of electrons passes across the surface and the secondary electrons are detected. Image produce is 3-D. The specimen is stained in heavy metals such as(osmium, lead, and gold). System is in a vacuum so there are no collisions with air particles restricting electrons getting to the surface of the specimen |
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