The gene machine synthesizes DNA fragments by translating a protein's amino acid sequence into a DNA sequence, creating oligonucleotides, assembling them, amplifying the gene via PCR, and inserting it into a plasmid.
The gene machine synthesizes DNA fragments by determining the amino acid sequence, finding complementary mRNA codons, designing oligonucleotides, assembling them automatically, replicating the gene using PCR, and inserting it into a plasmid.
| Front | Using gene machine to produce DNA fragments |
|---|---|
| Back | Amino acid sequence of desired protein determined mRNA codons are looked up and complementary DNA triplets determined Desired sequence of bases fed into computer Sequence checked for biosafety and biosecurity Computer designs oligonucleotides Oligonucleotides are assembled in an automated process which adds one nucleotide at a time Complementary strand of DNA is made and gene is replicated using PCR Gene is inserted into a plasmid for storage, cloning or transferring to other organisms |
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