| Front | Using gene machine to produce DNA fragments |
|---|---|
| Back | Amino acid sequence of desired protein determined mRNA codons are looked up and complementary DNA triplets determined Desired sequence of bases fed into computer Sequence checked for biosafety and biosecurity Computer designs oligonucleotides Oligonucleotides are assembled in an automated process which adds one nucleotide at a time Complementary strand of DNA is made and gene is replicated using PCR Gene is inserted into a plasmid for storage, cloning or transferring to other organisms |
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