Glucose boiling tubes calibration curve unknown make concentrations

Required Practical 11 involves creating a glucose dilution series, reacting samples with Benedict's solution in a water bath, measuring absorbance with a colorimeter, and plotting a calibration curve to determine unknown glucose concentrations.

Vereist Praktisch 11: Productie van een verdunningsreeks van een glucoseoplossing en gebruik van colorimetrische technieken om een kalibratiecurve te produceren waarmee de concentratie van glucose in een onbekend 'urine'-monster kan worden geïdentificeerd. 1. Maak een verdunningsreeks van glucose met gedestilleerd water. Gebruik zes reageerbuizen om concentraties te maken van 0 tot 10 mmol dm-3. 2. Plaats 2 cm³ van elk van de onbekende monsters in aparte reageerbuizen. 3. Voeg 2 cm³ Benedict's oplossing toe aan alle reageerbuizen. 4. Plaats reageerbuizen in een waterbad op 90°C gedurende vier minuten. 5. Gebruik een tang om de reageerbuizen uit het waterbad te halen. Laat afkoelen. 6. Nul de colorimeter met een cuvette met gedestilleerd water. De colorimeter moet worden ingesteld op het rode filter. 7. Plaats bekende monsters in de cuvette en meet de absorptie van elk met de colorimeter. 8. Maak een kalibratiecurve, waarbij de absorptie wordt uitgezet tegen de bekende glucoseconcentraties. 9. Meet de absorptie van de onbekende monsters met de colorimeter. Gebruik de kalibratiecurve om de glucoseconcentraties te bepalen.

Text	Required Practical 11:Production of a dilution series of a glucose solution and use of colorimetric techniques to produce a calibration curve with which to identify the concentration of glucose in an unknown ‘urine’ sample. 1. Create a dilution series of glucose using distilled water. Use six boiling tubes to make concentrations ranging from 0 to 10 mmol dm-3. 2. Place 2cm3 of each of the unknown samples in separate boiling tubes. 3. Add 2cm3 of Benedict’s solution to all boiling tubes. 4. Place boiling tubes in a water bath at 90°C for four minutes. 5. Use tongs to take the boiling tubes out of the water bath. Leave to cool. 6. Zero the colorimeter using a cuvette with distilled water. The colorimeter should be set to the red filter. 7. Place known samples into cuvette and measure the absorbance of each using the colorimeter. 8. Make a calibration curve, plotting the absorbance against the known glucose concentrations. 9. Measure the absorbance of the unknown samples using the colorimeter. Use the calibration curve to determine glucose concentrations

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Required Practical 11:Production of a dilution series of a glucose solution and use of colorimetric techniques to produce a calibration curve with which to identify the concentration of glucose in an unknown ‘urine’ sample.

1. Create a dilution series of glucose using distilled water. Use six boiling tubes to make concentrations ranging from 0 to 10 mmol dm-3.

2. Place 2cm3 of each of the unknown samples in separate boiling tubes.

3. Add 2cm3 of Benedict’s solution to all boiling tubes.

4. Place boiling tubes in a water bath at 90°C for four minutes.

5. Use tongs to take the boiling tubes out of the water bath. Leave to cool.

6. Zero the colorimeter using a cuvette with distilled water. The colorimeter should be set to the red filter.

7. Place known samples into cuvette and measure the absorbance of each using the colorimeter.

8. Make a calibration curve, plotting the absorbance against the known glucose concentrations.

9. Measure the absorbance of the unknown samples using the colorimeter. Use the calibration curve to determine glucose concentrations

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