Glucose boiling tubes calibration curve unknown make concentrations

Text	Required Practical 11:Production of a dilution series of a glucose solution and use of colorimetric techniques to produce a calibration curve with which to identify the concentration of glucose in an unknown ‘urine’ sample. 1. Create a dilution series of glucose using distilled water. Use six boiling tubes to make concentrations ranging from 0 to 10 mmol dm-3. 2. Place 2cm3 of each of the unknown samples in separate boiling tubes. 3. Add 2cm3 of Benedict’s solution to all boiling tubes. 4. Place boiling tubes in a water bath at 90°C for four minutes. 5. Use tongs to take the boiling tubes out of the water bath. Leave to cool. 6. Zero the colorimeter using a cuvette with distilled water. The colorimeter should be set to the red filter. 7. Place known samples into cuvette and measure the absorbance of each using the colorimeter. 8. Make a calibration curve, plotting the absorbance against the known glucose concentrations. 9. Measure the absorbance of the unknown samples using the colorimeter. Use the calibration curve to determine glucose concentrations

Text

Required Practical 11:Production of a dilution series of a glucose solution and use of colorimetric techniques to produce a calibration curve with which to identify the concentration of glucose in an unknown ‘urine’ sample.

1. Create a dilution series of glucose using distilled water. Use six boiling tubes to make concentrations ranging from 0 to 10 mmol dm-3.

2. Place 2cm3 of each of the unknown samples in separate boiling tubes.

3. Add 2cm3 of Benedict’s solution to all boiling tubes.

4. Place boiling tubes in a water bath at 90°C for four minutes.

5. Use tongs to take the boiling tubes out of the water bath. Leave to cool.

6. Zero the colorimeter using a cuvette with distilled water. The colorimeter should be set to the red filter.

7. Place known samples into cuvette and measure the absorbance of each using the colorimeter.

8. Make a calibration curve, plotting the absorbance against the known glucose concentrations.

9. Measure the absorbance of the unknown samples using the colorimeter. Use the calibration curve to determine glucose concentrations

Learn with these flashcards. Click next, previous, or up to navigate to more flashcards for this subject.