Cells are placed in a cold, buffered, isotonic solution to minimize enzymatic breakdown, prevent osmotic damage, and maintain stable pH and organelle structure.
Cells are kept cold to reduce enzyme activity, in an isotonic solution to prevent osmotic lysis or shrinkage, and in a buffered solution to maintain pH and organelle stability.
| Front | Why are cells placed in a cold buffered isotonic solution |
|---|---|
| Back | Cold- reduces enzyme activity which may break down organelle Same water potential- organelles prevented from bursting of shrinking due to change in osmotic balance Buffered- The pH is maintained so shape of organelle does not change |
Learn with these flashcards. Click next, previous, or up to navigate to more flashcards for this subject.
Next card: Stages cell fractionation homogenation ultracentrifugation
Previous card: Cell fractionation process broken separated
Up to card list: AQA Biology Flashcards