Sample remove place isolation add measure required practical

Text	Required Practical 8: Investigation into the effect of a named factor on the rate of dehydrogenase activity in extracts of chloroplasts 1. Remove stalks from leaf samples. Grind sample using a pestle and mortar and place into a chilled isolation solution. 2. Use a muslin cloth and funnel to filter the sample into a beaker. Suspend the beaker in an ice water bath to keep sample chilled. 3. Transfer to centrifuge tubes and centrifuge at high speed for 10 minutes. This will separate chloroplasts into the pellet. 4. Remove supernatant and add pellet to the fresh {{c4:isolation medium}}. Store isolation solution on ice. 5. Set the colorimeter to the red filter. Zero using a cuvette containing chloroplast extract and distilled water. 6. Place test tube in the rack 30cm from light source and add DCPIP. Immediately take a sample and add to cuvette. Measure the absorbance of the sample using the colorimeter. 7. Take a sample and measure its absorbance every 2 minutes for 10 minutes. 8. Repeat for different distances from lamp up to 100 cm. This will vary the light intensity.

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Required Practical 8: Investigation into the effect of a named factor on the rate of dehydrogenase activity in extracts of chloroplasts

1. Remove stalks from leaf samples. Grind sample using a pestle and mortar and place into a chilled isolation solution.

2. Use a muslin cloth and funnel to filter the sample into a beaker. Suspend the beaker in an ice water bath to keep sample chilled.

3. Transfer to centrifuge tubes and centrifuge at high speed for 10 minutes. This will separate chloroplasts into the pellet.

4. Remove supernatant and add pellet to the fresh {{c4:isolation medium}}. Store isolation solution on ice.

5. Set the colorimeter to the red filter. Zero using a cuvette containing chloroplast extract and distilled water.

6. Place test tube in the rack 30cm from light source and add DCPIP. Immediately take a sample and add to cuvette. Measure the absorbance of the sample using the colorimeter.

7. Take a sample and measure its absorbance every 2 minutes for 10 minutes.

8. Repeat for different distances from lamp up to 100 cm. This will vary the light intensity.

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