sterile bacteria plate dish agar tape required practical

Text	Required Practical 6: Aseptic technique method 1. Use a sterile pipette or wire hoop to transfer bacteria from broth (distilled water, bacterial culture, nutrients) to agar plate (petri dish containing agar jelly). 2. Spread bacteria evenly over plate using a sterile plastic spreader. 3. Use sterile forceps to place a multi disc antibiotic ring on the plate. Ring should only be moved by holding the centre, NOT the arms. 4. Lightly tape a lid on, invert and incubate at 25°C for 48 hours. DO NOT tape around the entire dish as this prevents oxygen entering and so promotes the growth of more harmful anaerobic bacteria. 5. Sterilise equipment used to handle bacteria and disinfect work surfaces. 6.Measure the diameter of the inhibition zone (clear circle) for each antibiotic. DO NOT remove the lid from the agar plate. 7.Work out the area of the inhibition zone

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Required Practical 6: Aseptic technique method

1. Use a sterile pipette or wire hoop to transfer bacteria from broth (distilled water, bacterial culture, nutrients) to agar plate (petri dish containing agar jelly).

2. Spread bacteria evenly over plate using a sterile plastic spreader.

3. Use sterile forceps to place a multi disc antibiotic ring on the plate. Ring should only be moved by holding the centre, NOT the arms.

4. Lightly tape a lid on, invert and incubate at 25°C for 48 hours. DO NOT tape around the entire dish as this prevents oxygen entering and so promotes the growth of more harmful anaerobic bacteria.

5. Sterilise equipment used to handle bacteria and disinfect work surfaces.

6.Measure the diameter of the inhibition zone (clear circle) for each antibiotic. DO NOT remove the lid from the agar plate.

7.Work out the area of the inhibition zone

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​Sterile Bacteria Plate Dish Agar Tape Required Practical