Increasing substrate concentration initially boosts the reaction rate as more enzyme molecules collide with substrates, but the rate eventually levels off when all enzyme active sites are occupied.
Increasing substrate concentration, with fixed enzyme concentration, initially increases the reaction rate due to more frequent collisions. However, the rate plateaus when all enzyme active sites are saturated.
| Front | How does substrate concentration affect enzyme activity? |
|---|---|
| Back | If the enzyme concentration is fixed, increasing the substrate concentration increases the rate of reaction. This is because at low substrate concentrations the enzyme molecules have only a limited number of substrate molecules to collide with however, after increasing the substrate concentration you increase the number of successful collisions that occur between enzymes and substrates. |
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