Test rate bath add of solution place tubes

Required Practical 9 investigates respiration rate in single-celled organisms by incubating yeast/glucose solutions at different temperatures (35-70°C), adding methylene blue, and timing its decolorization to calculate the rate.

Vereist Praktisch 9: Onderzoek naar het effect van een benoemde variabele op de reactiesnelheid van de ademhaling van culturen van eencellige organismen 1. Zet een waterbad op 35°C. 2. Voeg 5 cm³ van de gist en glucoseoplossing toe aan drie reageerbuizen. Plaats reageerbuizen in het waterbad en laat ze staan, zodat de oplossing gedurende 10 minuten kan equilibreren. 3. Voeg 2 cm³ methyleenblauw toe aan de reageerbuizen en start de timer. Schud 10 seconden en plaats reageerbuis terug in waterbad. Noteer hoe lang het duurt voordat het methyleenblauw naar elke reageerbuis kleurloos wordt. 4. Herhaal het experiment met temperaturen van 40°C, 50°C, 60°C en 70°C. 5. Vind het gemiddelde van de resultaten voor elke temperatuur en gebruik dit om de gemiddelde ademhalingssnelheid te berekenen.

Text	Required Practical 9:Investigation into the effect of a named variable on the rate of respiration of cultures of single-celled organisms 1. Set up a water bath at 35°C. 2. Add 5cm³ of the yeast and glucose solution to three test tubes. Place test tubes in the water bath and leave them, for the solution to equilibrate for 10 minutes. 3. Add 2cm³ of methylene blue to the test tubes and start the timer. Shake for 10 seconds and place test tube back in water bath. Record how long it takes for the methylene blue to turn colourless for each test tube. 4. Repeat the experiment using temperatures of 40°C, 50°C, 60°C and 70°C. 5. Find the mean of the results for each temperature and use to calculate the average rate of respiration.

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Required Practical 9:Investigation into the effect of a named variable on the rate of respiration of cultures of single-celled organisms

1. Set up a water bath at 35°C.

2. Add 5cm³ of the yeast and glucose solution to three test tubes. Place test tubes in the water bath and leave them, for the solution to equilibrate for 10 minutes.

3. Add 2cm³ of methylene blue to the test tubes and start the timer. Shake for 10 seconds and place test tube back in water bath. Record how long it takes for the methylene blue to turn colourless for each test tube.

4. Repeat the experiment using temperatures of 40°C, 50°C, 60°C and 70°C.

5. Find the mean of the results for each temperature and use to calculate the average rate of respiration.

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