Test rate bath add of solution place tubes

Text	Required Practical 9:Investigation into the effect of a named variable on the rate of respiration of cultures of single-celled organisms 1. Set up a water bath at 35°C. 2. Add 5cm³ of the yeast and glucose solution to three test tubes. Place test tubes in the water bath and leave them, for the solution to equilibrate for 10 minutes. 3. Add 2cm³ of methylene blue to the test tubes and start the timer. Shake for 10 seconds and place test tube back in water bath. Record how long it takes for the methylene blue to turn colourless for each test tube. 4. Repeat the experiment using temperatures of 40°C, 50°C, 60°C and 70°C. 5. Find the mean of the results for each temperature and use to calculate the average rate of respiration.

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Required Practical 9:Investigation into the effect of a named variable on the rate of respiration of cultures of single-celled organisms

1. Set up a water bath at 35°C.

2. Add 5cm³ of the yeast and glucose solution to three test tubes. Place test tubes in the water bath and leave them, for the solution to equilibrate for 10 minutes.

3. Add 2cm³ of methylene blue to the test tubes and start the timer. Shake for 10 seconds and place test tube back in water bath. Record how long it takes for the methylene blue to turn colourless for each test tube.

4. Repeat the experiment using temperatures of 40°C, 50°C, 60°C and 70°C.

5. Find the mean of the results for each temperature and use to calculate the average rate of respiration.

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