The ELISA test involves applying a sample to a slide to allow antigens to attach, washing away unbound antigens, adding a specific antibody that binds to the target antigen, washing again, adding an enzyme-linked antibody that binds to the first, and finally adding a substrate that produces a measurable color change.
- sample applied to slide - antigens attach to slide - unattached antigens washed off - antibody complementary to antigen being tested for added - binds to antigen - unattached antibodies washed off - second antibody attached to enzyme added - binds to first antibody - colourless substrate added - enzyme acts on substrate - coloured product produced - intensity of colour measured to determine antigen concentration (antigens, antibody 1, antibody 2 with enzyme, substrate)
| Front | How does the ELISA test work? |
|---|---|
| Back | - sample applied to slide - antigens attach to slide - unattached antigens washed off - antibody complementary to antigen being tested for added - binds to antigen - unattached antibodies washed off - second antibody attached to enzyme added - binds to first antibody - colourless substrate added - enzyme acts on substrate - coloured product produced - intensity of colour measured to determine antigen concentration (antigens, antibody 1, antibody 2 with enzyme, substrate) |
Tags: immunity
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