Ultracentrifugation separates organelles/proteins by density by spinning the homogenate at high speed, causing denser organelles to settle.
La ultracentrifugación separa orgánulos/proteínas por densidad al girar el homogeneizado a alta velocidad, haciendo que los orgánulos más densos se asienten.
| Front | Describe ultracentrifugation. |
|---|---|
| Back | - allows separation of organelles/proteins by density - generates centrifugal force - denser organelles settle at bottom of centrifuge tube - supernatant decanted, pellet at bottom removed - supernatant spun at higher speed to obtain next organelle |
Tags: microscopy
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