DNA probes, short labeled single strands of DNA with complementary sequences to target alleles, are used to locate specific genes by binding to denatured DNA through hybridization, detected by fluorescence or autoradiography.
- to locate specific alleles of genes - short single-strand of DNA - labelled - radioactive/fluorescent - complementary base sequence to target allele - DNA sample being tested is heated - to break H bonds and separate strands - DNA probe binds to complementary base sequence by DNA hybridisation - viweing fluoresence or autoradiography is used to identify allele of gene
| Front | How are DNA probes used? |
|---|---|
| Back | - to locate specific alleles of genes - short single-strand of DNA - labelled - radioactive/fluorescent - complementary base sequence to target allele - DNA sample being tested is heated - to break H bonds and separate strands - DNA probe binds to complementary base sequence by DNA hybridisation - viweing fluoresence or autoradiography is used to identify allele of gene |
Tags: gene_technology
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