Desired genes can be isolated for recombinant DNA technology by using reverse transcriptase to create cDNA from mRNA, cutting genes from chromosomes with restriction enzymes, or synthesizing them artificially using a gene machine.
- isolate mRNA produced from desired gene from cell - use reverse transciptase to catalyse production of single strand of DNA - use DNA polymerase to form double strand complementary DNA - cut desired gene from chromosome - using specific restriction endonuclease with complementary recognition sequence - produces sticky ends - make gene with desired base sequence artificially - using gene machine and DNA polymerase
| Front | What are the three ways to cut DNA fragments and isolate genes? |
|---|---|
| Back | - isolate mRNA produced from desired gene from cell - use reverse transciptase to catalyse production of single strand of DNA - use DNA polymerase to form double strand complementary DNA - cut desired gene from chromosome - using specific restriction endonuclease with complementary recognition sequence - produces sticky ends - make gene with desired base sequence artificially - using gene machine and DNA polymerase |
Tags: gene_technology
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