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Dna Separate Gel Compare Genetic Fingerprints Cut Restriction

Front How do you compare genetic fingerprints?
Back - cut DNA using restriction endonuclease
- clone DNA using PCR

- separate DNA molecules by length using gel electrophoresis
- immerse gel in alkali to break H bonds and separate DNA strands

- transfer DNA to nylon membrane by southern blotting
- use radioactive DNA probes with complementary base sequences to VNTRs
- use autoradiography to view banding pattern

Tags: gene_technology

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