Genetic fingerprinting involves cutting DNA with restriction enzymes, PCR amplification, gel electrophoresis to separate fragments by size, Southern blotting, and then using radioactive DNA probes complementary to VNTRs, visualized by autoradiography.
- cut DNA using restriction endonuclease - clone DNA using PCR - separate DNA molecules by length using gel electrophoresis - immerse gel in alkali to break H bonds and separate DNA strands - transfer DNA to nylon membrane by southern blotting - use radioactive DNA probes with complementary base sequences to VNTRs - use autoradiography to view banding pattern
| Front | How do you compare genetic fingerprints? |
|---|---|
| Back | - cut DNA using restriction endonuclease - clone DNA using PCR - separate DNA molecules by length using gel electrophoresis - immerse gel in alkali to break H bonds and separate DNA strands - transfer DNA to nylon membrane by southern blotting - use radioactive DNA probes with complementary base sequences to VNTRs - use autoradiography to view banding pattern |
Tags: gene_technology
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