To test for a non-reducing sugar, first heat with Benedict's solution; if negative, hydrolyze with acid, neutralize with alkali, and re-test with Benedict's, looking for a brick-red precipitate.
- heat with benedict's solution - if no colour change, reducing sugar is not present - heat with hydrochloric acid to hydrolyse into monosaccharides - add sodium hydrogencarbonate until solution is alkaline - heat with benedict's solution - colour change from blue solution to brick red precipitate
| Front | How do you test for a non-reducing sugar? |
|---|---|
| Back | - heat with benedict's solution - if no colour change, reducing sugar is not present - heat with hydrochloric acid to hydrolyse into monosaccharides - add sodium hydrogencarbonate until solution is alkaline - heat with benedict's solution - colour change from blue solution to brick red precipitate |
Tags: carbohydrates
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