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Growth Plant Shoot Sterile Stages Tissue Culture Breeding

The seven stages of tissue culture breeding are: choosing a plant with desirable characteristics, taking cells from the shoot tip using sterile forceps, placing them on a nutrient growth medium with hormones, incubating at 30°C, acclimatizing in a greenhouse, allowing growth and maturation, and finally planting outdoors.

Los siete pasos del cultivo de tejidos son: selección de planta con características deseables, extracción de células del ápice del brote en condiciones estériles, colocación en medio de crecimiento nutritivo con hormonas, incubación a 30°C, aclimatación en invernadero, crecimiento y maduración, y finalmente, trasplante al exterior.

Front
What are the seven stages of tissue culture breeding?
Back
1. Plant with desirable characteristics chosen
2. Cells taken from shoot tip with sterile forceps
3. Sterile conditions
4. Placed on nutrients growth medium containing growth hormones. To encourage shoot + root growth
5. Incubate at 30°C
6. Greenhouse to acclimatise grow and mature
7. Plant outside

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