The five key stages for the aseptic technique when inoculating a wire loop involve: sterilizing the petri dish (nutrient agar) at 120°C, sterilizing the inoculating loop by heating it in a Bunsen flame before and after transferring microorganisms, using the cooled loop to collect microorganisms from a culture, transferring these microorganisms to the nutrient agar by slightly opening the petri dish lid, and finally sealing the petri dish lid with adhesive tape to prevent airborne contamination.
The five key stages for the aseptic technique of inoculating a wire loop are: sterilizing the petri dish, sterilizing the inoculating loop before and after transfer, collecting microorganisms with the cooled loop, transferring them to the agar while minimally opening the dish lid, and sealing the lid to prevent contamination.
| Front | What are the five key stages to the aseptic technique (inoculating a wire loop)? |
|---|---|
| Back | 1. The petri dish with nutrient agar is sterilised by heating to 120°C
2. An inoculating loop is sterilised by heating in a Bunsen flame before and after transferring microorganisms
3. The cooled inoculating loop is used to collect microorganisms from a culture
4. The lid of the petri dish is raised just sufficiently to allow microorganisms to be transferred from the inoculating loop to the nutrient agar
5. The lid of the petri dish is sealed with adhesive tape to prevent contamination by microorganisms from the air |
Learn with these flashcards. Click next, previous, or up to navigate to more flashcards for this subject.
Next card: Inoculating loop heated hot bunsen flame sterilise disinfect
Previous card: Asepsis absence unwanted organisms what
Up to card list: Biology Topics 1-9 GCSE