| Front | What are the five key stages to the aseptic technique (inoculating a wire loop)? |
|---|---|
| Back | 1. The petri dish with nutrient agar is sterilised by heating to 120°C
2. An inoculating loop is sterilised by heating in a Bunsen flame before and after transferring microorganisms
3. The cooled inoculating loop is used to collect microorganisms from a culture
4. The lid of the petri dish is raised just sufficiently to allow microorganisms to be transferred from the inoculating loop to the nutrient agar
5. The lid of the petri dish is sealed with adhesive tape to prevent contamination by microorganisms from the air |
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