Question

[[oc1::1) Place a drop of iodine solution into every well of a spotting tile]][[oc2::2) Use a syringe to add 1cm3 of amylase solution and 1 cm3 of buffer solution with a pH of 5 to a boiling tube and using test tube holders, place it into a water bath set to 35ºC and wait 5 minutes]][[oc3::3) Use a different syrine to add 5cm3 of a starch solution to the boiling tube]][[oc4::4) Immediately mix contents of the boiling tube and start a stopwatch]][[oc5::5) Use continuous sampling to record how long it takes for starch to be broken down by amylase by putting a drop into the well every 30 seconds - when the iodine remains browny-orange starch is not present]][[oc6::6) Repeat the whole experiment with buffer solutions of different pH values to see how pH affects the time taken for starch to be broken down]] 1,1,0 | n,n,n,y 1) Place a drop of iodine solution into every well of a spotting tile2) Use a syringe to add 1cm3 of amylase solution and 1 cm3 of buffer solution with a pH of 5 to a boiling tube and using test tube holders, place it into a water bath set to 35ºC and wait 5 minutes............ ......3) Use a different syrine to add 5cm3 of a starch solution to the boiling tube4) Immediately mix contents of the boiling tube and start a stopwatch...... ............5) Use continuous sampling to record how long it takes for starch to be broken down by amylase by putting a drop into the well every 30 seconds - when the iodine remains browny-orange starch is not present6) Repeat the whole experiment with buffer solutions of different pH values to see how pH affects the time taken for starch to be broken down

Accepted Answer

Investigating the Effect of pH on Enzyme Activity 1) Place a drop of iodine solution into every well of a spotting tile............... ...2) Use a syringe to add 1cm3 of amylase solution and 1 cm3 of buffer solution with a pH of 5 to a boiling tube and using test tube holders, place it into a water bath set to 35ºC and wait 5 minutes3) Use a different syrine to add 5cm3 of a starch solution to the boiling tube......... .........4) Immediately mix contents of the boiling tube and start a stopwatch5) Use continuous sampling to record how long it takes for starch to be broken down by amylase by putting a drop into the well every 30 seconds - when the iodine remains browny-orange starch is not present...

Original	[[oc1::1) Place a drop of iodine solution into every well of a spotting tile]] [[oc2::2) Use a syringe to add 1cm³ of amylase solution and 1 cm³ of buffer solution with a pH of 5 to a boiling tube and using test tube holders, place it into a water bath set to 35ºC and wait 5 minutes]] [[oc3::3) Use a different syrine to add 5cm³ of a starch solution to the boiling tube]] [[oc4::4) Immediately mix contents of the boiling tube and start a stopwatch]] [[oc5::5) Use continuous sampling to record how long it takes for starch to be broken down by amylase by putting a drop into the well every 30 seconds - when the iodine remains browny-orange starch is not present]] [[oc6::6) Repeat the whole experiment with buffer solutions of different pH values to see how pH affects the time taken for starch to be broken down]]
Title	Investigating the Effect of pH on Enzyme Activity
Settings	1,1,0 \| n,n,n,y
Text1	1) Place a drop of iodine solution into every well of a spotting tile ... ... ... ... ...
Text2	1) Place a drop of iodine solution into every well of a spotting tile 2) Use a syringe to add 1cm³ of amylase solution and 1 cm³ of buffer solution with a pH of 5 to a boiling tube and using test tube holders, place it into a water bath set to 35ºC and wait 5 minutes ... ... ... ...
Text3	... 2) Use a syringe to add 1cm³ of amylase solution and 1 cm³ of buffer solution with a pH of 5 to a boiling tube and using test tube holders, place it into a water bath set to 35ºC and wait 5 minutes 3) Use a different syrine to add 5cm³ of a starch solution to the boiling tube ... ... ...
Text4	... ... 3) Use a different syrine to add 5cm³ of a starch solution to the boiling tube 4) Immediately mix contents of the boiling tube and start a stopwatch ... ...
Text5	... ... ... 4) Immediately mix contents of the boiling tube and start a stopwatch 5) Use continuous sampling to record how long it takes for starch to be broken down by amylase by putting a drop into the well every 30 seconds - when the iodine remains browny-orange starch is not present ...
Text6	... ... ... ... 5) Use continuous sampling to record how long it takes for starch to be broken down by amylase by putting a drop into the well every 30 seconds - when the iodine remains browny-orange starch is not present 6) Repeat the whole experiment with buffer solutions of different pH values to see how pH affects the time taken for starch to be broken down

Solution Tube Starch Boiling Ph Place Drop Iodine