The experiment investigates the effect of pH on enzyme activity, starting with adding iodine to a spotting tile, then mixing amylase and a pH 5 buffer solution in a water bath, adding starch, and timing starch breakdown using continuous sampling with iodine.
El experimento investiga el efecto del pH en la actividad enzimática, comenzando con la adición de yodo a una placa de detección, luego mezclando amilasa y una solución tampón de pH 5 en un baño de agua, añadiendo almidón, y cronometrando la descomposición del almidón mediante muestreo continuo con yodo.
| Original | [[oc1::1) Place a drop of iodine solution into every well of a spotting tile]] [[oc2::2) Use a syringe to add 1cm3 of amylase solution and 1 cm3 of buffer solution with a pH of 5 to a boiling tube and using test tube holders, place it into a water bath set to 35ºC and wait 5 minutes]] [[oc3::3) Use a different syrine to add 5cm3 of a starch solution to the boiling tube]] [[oc4::4) Immediately mix contents of the boiling tube and start a stopwatch]] [[oc5::5) Use continuous sampling to record how long it takes for starch to be broken down by amylase by putting a drop into the well every 30 seconds - when the iodine remains browny-orange starch is not present]] [[oc6::6) Repeat the whole experiment with buffer solutions of different pH values to see how pH affects the time taken for starch to be broken down]] |
|---|---|
| Title | Investigating the Effect of pH on Enzyme Activity |
| Settings | 1,1,0 | n,n,n,y |
| Text1 | 1) Place a drop of iodine solution into every well of a spotting tile ... ... ... ... ... |
| Text2 | 1) Place a drop of iodine solution into every well of a spotting tile 2) Use a syringe to add 1cm3 of amylase solution and 1 cm3 of buffer solution with a pH of 5 to a boiling tube and using test tube holders, place it into a water bath set to 35ºC and wait 5 minutes ... ... ... ... |
| Text3 | ... 2) Use a syringe to add 1cm3 of amylase solution and 1 cm3 of buffer solution with a pH of 5 to a boiling tube and using test tube holders, place it into a water bath set to 35ºC and wait 5 minutes 3) Use a different syrine to add 5cm3 of a starch solution to the boiling tube ... ... ... |
| Text4 | ... ... 3) Use a different syrine to add 5cm3 of a starch solution to the boiling tube 4) Immediately mix contents of the boiling tube and start a stopwatch ... ... |
| Text5 | ... ... ... 4) Immediately mix contents of the boiling tube and start a stopwatch 5) Use continuous sampling to record how long it takes for starch to be broken down by amylase by putting a drop into the well every 30 seconds - when the iodine remains browny-orange starch is not present ... |
| Text6 | ... ... ... ... 5) Use continuous sampling to record how long it takes for starch to be broken down by amylase by putting a drop into the well every 30 seconds - when the iodine remains browny-orange starch is not present 6) Repeat the whole experiment with buffer solutions of different pH values to see how pH affects the time taken for starch to be broken down |
Tags: b2, bio, enzymes-digestion
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