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GCSE Biology Paper 1 (9-1 Exams)

Solution Ph Boiling Cm Starch Tube Put Drop

Front List the steps needed in detail to investigate the effect of pH on amylase.
Back
  1. Put a drop of iodine solution into every well of a spotting tile.
  2. Use a syringe to add 1 cm3 of amylase solution and 1 cm3 of a bufer solution with a pH 5 to a boiling tube.
  3. Use a different syringe to add 5 cm3 of a starch solution to the boiling tube
  4. Mix the contents of the boiling tube and start a stop clock
  5. Use a dropping pipette to take a ffresh sample from the boiling tube every thirty seconds and put a drop into a well. When the iodine solution remains browny-orange, starch is no longer present.
  6. Repeat the experiment with buffer solutions of different pH values to see how pH affects the time taken for the starch to be broken down
  7. Control variables include concentration and volume of amylase solution to make it a fair test.

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