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GCSE Biology Paper 1 (9-1 Exams)

Solution Ph Boiling Cm Starch Tube Put Drop

To investigate the effect of pH on amylase, place iodine drops in a spotting tile. Add amylase and buffer solutions of different pH values to boiling tubes containing starch solution. Mix, start a clock, and periodically take samples to test with iodine. Record the time until the iodine remains browny-orange, indicating starch breakdown. Control variables like amylase concentration and volume for a fair test.

Per studiare l'effetto del pH sull'amilasi, si mettono gocce di iodio nelle buche di una piastra a reagenti, si aggiungono amilasi e tamponi di pH diversi a tubi contenenti amido, si mescola, si prelevano campioni a intervalli regolari con una pipetta e si aggiungono alle buche. Si registra il tempo necessario affinché lo iodio rimanga arancione-marrone, indicando che l'amido è stato scomposto. Le variabili controllate includono la concentrazione e il volume dell'amilasi.

Front List the steps needed in detail to investigate the effect of pH on amylase.
Back
  1. Put a drop of iodine solution into every well of a spotting tile.
  2. Use a syringe to add 1 cm3 of amylase solution and 1 cm3 of a bufer solution with a pH 5 to a boiling tube.
  3. Use a different syringe to add 5 cm3 of a starch solution to the boiling tube
  4. Mix the contents of the boiling tube and start a stop clock
  5. Use a dropping pipette to take a ffresh sample from the boiling tube every thirty seconds and put a drop into a well. When the iodine solution remains browny-orange, starch is no longer present.
  6. Repeat the experiment with buffer solutions of different pH values to see how pH affects the time taken for the starch to be broken down
  7. Control variables include concentration and volume of amylase solution to make it a fair test.

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