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IB Biology HL

Thymidine Silver Bromide Cells Tritiated Chromosomes Surface Grains

Original
1. Cells are grown in a solution containing radioactive thymidine (tritiated thymidine – 3H-T)
2. The tritiated thymidine is incorporated into the chromosomal DNA of the cell (3H-T is used as thymidine is not present in RNA)
3. The chromosomes are isolated by gently lysing the cells and fixing the chromosomes to a photographic surface
4. The surface is then immersed in a radioactively-sensitive emulsion containing silver bromide (AgBr)
5. The radiation released from the tritiated thymidine converts the Ag+ ions in silver bromide into insoluble metal grains
6. Following a period of exposure, excess silver bromide is washed away, leaving the silver grains to appear as small black dots
7. When the photographic film is developed, the chromosomal DNA can be visualised with an electron microscope
Title Process of autoradiography
Settings 1,1,0 | n,n,n,n
Text1
1. Cells are grown in a solution containing radioactive thymidine (tritiated thymidine – 3H-T)
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Text2
1. Cells are grown in a solution containing radioactive thymidine (tritiated thymidine – 3H-T)
2. The tritiated thymidine is incorporated into the chromosomal DNA of the cell (3H-T is used as thymidine is not present in RNA)
...
...
...
...
...
Text3
...
2. The tritiated thymidine is incorporated into the chromosomal DNA of the cell (3H-T is used as thymidine is not present in RNA)
3. The chromosomes are isolated by gently lysing the cells and fixing the chromosomes to a photographic surface
...
...
...
...
Text4
...
...
3. The chromosomes are isolated by gently lysing the cells and fixing the chromosomes to a photographic surface
4. The surface is then immersed in a radioactively-sensitive emulsion containing silver bromide (AgBr)
...
...
...
Text5
...
...
...
4. The surface is then immersed in a radioactively-sensitive emulsion containing silver bromide (AgBr)
5. The radiation released from the tritiated thymidine converts the Ag+ ions in silver bromide into insoluble metal grains
...
...
Text6
...
...
...
...
5. The radiation released from the tritiated thymidine converts the Ag+ ions in silver bromide into insoluble metal grains
6. Following a period of exposure, excess silver bromide is washed away, leaving the silver grains to appear as small black dots
...
Text7
...
...
...
...
...
6. Following a period of exposure, excess silver bromide is washed away, leaving the silver grains to appear as small black dots
7. When the photographic film is developed, the chromosomal DNA can be visualised with an electron microscope
Full
1. Cells are grown in a solution containing radioactive thymidine (tritiated thymidine – 3H-T)
2. The tritiated thymidine is incorporated into the chromosomal DNA of the cell (3H-T is used as thymidine is not present in RNA)
3. The chromosomes are isolated by gently lysing the cells and fixing the chromosomes to a photographic surface
4. The surface is then immersed in a radioactively-sensitive emulsion containing silver bromide (AgBr)
5. The radiation released from the tritiated thymidine converts the Ag+ ions in silver bromide into insoluble metal grains
6. Following a period of exposure, excess silver bromide is washed away, leaving the silver grains to appear as small black dots
7. When the photographic film is developed, the chromosomal DNA can be visualised with an electron microscope

Tags: 3_2_chromosomes

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