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Plasmid Desired Gene Restriction Forming Sticky Ends Bacterial

Front What is the process of genetic engineering?
Back Isolation of the desired gene using restriction enzymes, forming sticky ends,
Cutting of bacterial plasmid DNA with the same restriction enzyme, forming complementary sticky ends,
Insertion of desired gene into bacterial plasmid DNA using ligase to form a recombinant plasmid,
Insertion of plasmid into bacteria,
Replication of bacteria in a fermenter to produce bacteria containing the recombinant plasmid DNA.

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