Isolation of the desired gene using restriction enzymes, forming sticky ends; cutting of bacterial plasmid DNA with the same restriction enzyme, forming complementary sticky ends; insertion of desired gene into bacterial plasmid DNA using ligase to form a recombinant plasmid; insertion of plasmid into bacteria; replication of bacteria in a fermenter to produce bacteria containing the recombinant plasmid DNA.
Isolering af det ønskede gen ved restriktionsenzymer, dannelse af 'sticky ends'; klipning af bakteriel plasmid-DNA med samme restriktionsenzym, dannelse af komplementære 'sticky ends'; indsættelse af ønsket gen i bakteriel plasmid-DNA ved ligase for at danne et rekombinant plasmid; indsættelse af plasmid i bakterier; replikation af bakterier i en fermentor for at producere bakterier indeholdende det rekombinante plasmid-DNA.
| Front | What is the process of genetic engineering? |
|---|---|
| Back | Isolation of the desired gene using restriction enzymes, forming sticky ends, Cutting of bacterial plasmid DNA with the same restriction enzyme, forming complementary sticky ends, Insertion of desired gene into bacterial plasmid DNA using ligase to form a recombinant plasmid, Insertion of plasmid into bacteria, Replication of bacteria in a fermenter to produce bacteria containing the recombinant plasmid DNA. |
Learn with these flashcards. Click next, previous, or up to navigate to more flashcards for this subject.
Next card: Enzymes restriction cut dna specific sites
Previous card: Make common yeast alcohol bread
Up to card list: IGCSE Biology Summary