Cutting DNA at specific locations involves incubating purified DNA with restriction enzymes. Progression is checked by gel electrophoresis. The gene of interest and the vector are mixed, and ligase is added.
El corte de ADN se realiza incubando ADN purificado con enzimas de restricción. La progresión se verifica mediante electroforesis en gel. El gen de interés y el vector se mezclan y se añade ligasa.
| Front | Cutting of DNA at specific Location |
|---|---|
| Back | -incubate purified DNA with restriction enzyme at optimal temperature -Agarose gel electrophorosis is emplyed for progression -interest gene and the vector are mixed and ligase is added |
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