DNA is hydrophilic and doesn't easily pass through the cell membrane. To make bacterial cells competent, they are treated with a specific concentration of a divalent cation, incubated with DNA on ice, then briefly placed at 42°C, and put back on ice.
El ADN es hidrofílico y no atraviesa fácilmente la membrana celular. Para hacer competentes a las células bacterianas, se tratan con cationes divalentes, se incuban con ADN en hielo, luego se exponen brevemente a 42°C y se vuelven a colocar en hielo.
| Front | Making host competetnt |
|---|---|
| Back | dna is hydrophilic -no passage through cell membrane so bacterial cel are treated with a specific con of a divalent cation-DNA enters through pores -incubated with DNA on ice .then place briefly on 42 and put them back on ice |
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