This flashcard is about the Meselson and Stahl experiment, detailing the organism used (E. coli), the medium containing heavy nitrogen (¹⁵NH₄Cl), separation of DNA via CsCl density gradient centrifugation, and a cycle duration of 20 minutes.
此闪卡是关于 Meselson 和 Stahl 实验的，实验过程中使用了大肠杆菌（E.coli）、含¹⁵N氨化铵的培养基，并通过CsCl密度梯度离心区分DNA，每个周期时长20分钟。
| Subject | messelson and stahl experiment |
|---|---|
| Q1 | organism grown |
| Q1 Answer | E.coli |
| Q2 | medium containing |
| Q2 Answer | 15NH4Cl (heavy isotope of nitrogen) |
| Q3 | how could the normal dna be distinguished |
| Q3 Answer | centrifugation with CsCl density gradient |
| Q4 | duration of each cycle |
| Q4 Answer | 20 minutes |
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