In PCR for diagnosis, ssDNA or ssRNA is tagged and allowed to hybridize with complementary DNA. If mutated, it won't be visible in autoradiography.
En la PCR diagnóstica, el ADN o ARN de cadena simple se marca y se permite que se hibride con el ADN complementario. Si hay mutación, no se puede observar en la autorradiografía.
Front | PCR is done |
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Back | the ssDNA or ssRNA is tagged and then allowed to hybridise with the complementary DNA is it is mutated then in autoradiography we cant see it |
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