DNA fingerprinting involves producing RFLPs, performing agarose gel electrophoresis, transferring the pattern to a membrane, denaturing the DNA, hybridizing with radioactive probes, and obtaining an autoradiograph.
La huella dactilar de ADN implica producir RFLPs, realizar electroforesis en gel de agarosa, transferir el patrón a una membrana, desnaturalizar el ADN, hibridar con sondas radiactivas y obtener una autoradiografía.
Front | DNA Fingerprinting procedure |
---|---|
Back | 1) Produce RFLPS (restriction fragment length polymorphism) using restriction enzymes. 2) Perform electrophoresis in agarose gel. 3) Transfer the gel pattern to a membrane and denature the DNA. 4) Hybridize the DNA with radioactive probes and obtain autoradiograph. |
Learn with these flashcards. Click next, previous, or up to navigate to more flashcards for this subject.
Next card: Model plant arabidopsis name of
Previous card: Dna sequencing/analysis identify parents criminals diagnose viral infections
Up to card list: nMDCAT Biology (Pakistan)