Gram-positive bacteria appear purple due to retaining the crystal violet-iodine complex, while Gram-negative bacteria appear pink after retaining the secondary dye.
Gram-positive bacteria stain purple because they retain the crystal violet-iodine complex, while Gram-negative bacteria stain pink as they retain the counterstain.
| Front | Staining of bacteria wrt cell wall type |
|---|---|
| Back | Gram +ve: purple retain primary dye (CV-1 complex) Gram -ve: pink retain secondary dye |
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