The steps of PCR are: 1) Heat to 94-96°C for 1 minute to denature DNA, 2) Cool for 2 minutes for primer annealing, and 3) Add primers and Taq polymerase, waiting 1.5 minutes for extension, then repeating the cycle.
Los pasos de la PCR son: 1) Calentar a 94-96°C durante 1 minuto para desnaturalizar el ADN, 2) Enfriar durante 2 minutos para la unión de cebadores, y 3) Añadir cebadores y Taq polimerasa, esperando 1.5 minutos para la extensión, repitiendo el ciclo.
| Front | PCR steps |
|---|---|
| Back | 1) Heat for 1 minute to denature 2) Cool for 2 minutes 3) Add primers and Taq polymerase, and wait for 1.5 minutes -> Repeat |
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