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Cube Student Liver Frozen Carried Investigation Catalase Procedure

The frozen and thawed liver cube bubbles more than the refrigerated one because freezing and thawing damages the cell membrane, increasing permeability to hydrogen peroxide and catalase, thus allowing more hydrogen peroxide to be broken down.

Külmkapis hoitud maksa kuubikuga võrreldes külmutatud ja sulatatud maksa kuubik rohkem vahutab, sest külmutamine ja sulatamine kahjustab rakumembraani, suurendades selle läbilaskvust vesinikperoksiidile ja katalaasile. See võimaldab rohkem vesinikperoksiidi lagundada.

Front A student carried out an investigation on catalase using the following procedure:
• two identical sized cubes were cut from a piece of fresh liver
• one cube was frozen overnight and then defrosted
• the other cube was stored in the refrigerator
• both cubes were returned to room temperature and were placed in separate test tubes containing equal volumes of 2% hydrogen peroxide solution.

The student observed that the cube of liver that had been frozen and defrosted, bubbled
significantly more than the cube that had been refrigerated.
Suggest an explanation for this result.
Back freezing/defrosting damages cell membrane, inc permeability of membrane to H2O2/catalase, so more H2O2 broken down

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