The flashcard outlines the steps for DNA extraction: grinding the sample, mixing with detergent to break down membranes, adding salt to disrupt hydrogen bonds, using protease to remove associated proteins, precipitating DNA with alcohol, and collecting the DNA.
Uzmala, oštetila uzorak za razgradnju staničnih stijenki, pomiješala uzorak s deterdžentom za razgradnju stanične membrane (stanični sadržaj pušten u otopinu), dodala sol za razgradnju H2 veza između DNK i vode, dodala proteazu koja razgrađuje proteine povezane s DNK, dodala sloj alkohola na vrh kako bi se DNK istaložila kao bijeli talog (slojevita kao uzorak i alkohol), pokupila DNK namatanjem na stakleni štap
| Front | Extracting DNA 2.1.3(d) |
|---|---|
| Back | grind sample to break down cell walls, mix sample with detergent to break down cell membrane (cell contents release into sol), add salt to break H2 bonds between DNA/water, add protease which breaks down proteins associated with DNA, add layer of alcohol on top so DNA precipitates out as white ppt (layer between sample and alcohol), pick up DNA by spooling onto glass rod |
Learn with these flashcards. Click next, previous, or up to navigate to more flashcards for this subject.
Next card: Competitive examples non-competitive inhibition 2.1.4(f statins inhibit enzymes
Previous card: Sample prep 2.1.1(b
Up to card list: OCR A Biology