Question

inoculating agar

Accepted Answer

inoculating agar

wire inoculating loop sterilised by holding close to flame until glows red hot
cool and dip in bacterial suspension
remove petri dish lid and streak loop horizontally across agar surface in zig-zag pattern
replace lid and hold with tape (not fully sealed to allow O2 to enter and prevent formation of anaerobic bacteria) and incubate at set temp

Front	inoculating agar
Back	inoculating agar wire inoculating loop sterilised by holding close to flame until glows red hot cool and dip in bacterial suspension remove petri dish lid and streak loop horizontally across agar surface in zig-zag pattern replace lid and hold with tape (not fully sealed to allow O2 to enter and prevent formation of anaerobic bacteria) and incubate at set temp

Inoculating Agar Loop Lid Wire Sterilised Holding Close