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OCR A Biology

Inoculating Agar Loop Lid Wire Sterilised Holding Close

Inoculating agar involves sterilizing an inoculating loop, dipping it into a bacterial suspension, and streaking it onto the agar surface in a Petri dish, followed by incubation.

A inoculação de agar envolve esterilizar um anel de inoculação, mergulhá-lo em uma suspensão bacteriana e estriá-lo na superfície do agar em um prato de Petri, seguido pela incubação.

Front inoculating agar
Back
  • inoculating agar
    • wire inoculating loop sterilised by holding close to flame until glows red hot
    • cool and dip in bacterial suspension
    • remove petri dish lid and streak loop horizontally across agar surface in zig-zag pattern
    • replace lid and hold with tape (not fully sealed to allow O2 to enter and prevent formation of anaerobic bacteria) and incubate at set temp

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