Laser scanning confocal microscopy uses a laser for illumination, a mirror to direct specific wavelengths, scans the specimen point by point to detect fluorescence, and a pinhole aperture to exclude out-of-focus light, resulting in high-resolution 2D images.
Laser scanning confocal microscopy uses a laser for illumination, a mirror to direct specific wavelengths, scans the specimen point by point to detect fluorescence, and a pinhole aperture to exclude out-of-focus light, resulting in high-resolution 2D images.
| Front | Laser scanning conforcal microscopy 2.1.1(a) |
|---|---|
| Back | -light emitted from laser(higher intensity than light) -mirror reflects rays of only one wavelength(others transmitted) towards objective lens reflecting to sample -single spot of focused light moved across specimen, causing florescence(absorption and re-emission of light, re-emitted at longer wavelength to produce magnified image) -emmited light follows same path as lasers rays but towards detector -only light radiated from near focal plane(distance with sharpest distance) detected and unwanted light doesn't pass through pinhole aperture -high res as thin section used, 2D image |
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