Plant Tissue Culture Medium Hormones Outline Steps Micropropagation

Micropropagation involves taking plant tissue, sterilizing it, placing it in a culture medium with hormones to stimulate mitosis, growing callus, developing plantlets, and finally potting them into compost.

A micropropagação envolve a coleta de tecido vegetal, esterilização, colocação em meio de cultura com hormônios para estimular a mitose, crescimento de calos, desenvolvimento de plântulas e, finalmente, o plantio em composto.

Front

Outline the steps in micropropagation of a plant

Back

Take some tissue from the shoots or roots of a plant (meristematic)
Sterilised the sample (prevent the plant tissue from becoming contaminated with bacteria or fungi)
Place the explant (material being removed from parent plant) in sterile culture medium containing plant hormones which stimulate mitosis
Mass of undifferentiated cells (callus) is divided and put into new culture medium (with hormones and nutrients) which stimulates plantlet growth
Plantlets are potted into compost

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