Plant Tissue Culture Medium Hormones Outline Steps Micropropagation

Outline the steps in micropropagation of a plant

• Take some tissue from the shoots or roots of a plant (meristematic)
• Sterilised the sample (prevent the plant tissue from becoming contaminated with bacteria or fungi)
• Place the explant (material being removed from parent plant) in sterile culture medium containing plant hormones which stimulate mitosis
• Mass of undifferentiated cells (callus) is divided and put into new culture medium (with hormones and nutrients) which stimulates plantlet growth
• Plantlets are potted into compost