Conc Solution Factors Enzyme Controlled Add Hydrogen Peroxide Describe

Enzyme activity can be investigated using catalase and hydrogen peroxide, measuring oxygen production while manipulating substrate concentration, enzyme concentration, temperature, or pH.

Enzyme-controlled reactions can be investigated using catalase and hydrogen peroxide, measuring oxygen production, and altering variables like substrate concentration, enzyme concentration, temperature, and pH.

Front

Describe and explain how to investigate any of the factors that affect the rate of enzyme-controlled reactions.

Back

Investigating factors affecting enzyme-controlled reactions:

Use catalase from any living tissue and add it to hydrogen peroxide, measure gas produced (oxygen):

Changing the ind. variables:

Substrate conc - increase hydrogen peroxide conc

Enzyme conc - eg crush up potatoes in solution and perform serial dilution of the solution

Temperature - heat the tissue/perform experiment in water bath where everything is at same temp

pH - add buffer solution to the reaction mixture

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