Conc Solution Factors Enzyme Controlled Add Hydrogen Peroxide Describe

Front

Describe and explain how to investigate any of the factors that affect the rate of enzyme-controlled reactions.

Back

Investigating factors affecting enzyme-controlled reactions:

Use catalase from any living tissue and add it to hydrogen peroxide, measure gas produced (oxygen):

Changing the ind. variables:

Substrate conc - increase hydrogen peroxide conc

Enzyme conc - eg crush up potatoes in solution and perform serial dilution of the solution

Temperature - heat the tissue/perform experiment in water bath where everything is at same temp

pH - add buffer solution to the reaction mixture

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